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Method of Non-Chromatographic Purification of Monoclonal Antibodies
The potent pharmacodynamics and numerous diagnostics of monoclonal antibodies (mAbs) have led to their extensive use as therapeutic treatments for various cancers and autoimmune disorders, and for diagnostic assays. However, rapid developments in the foundational cell biology and recombinant technology of mAbs have not been matched by progress in downstream purification, a major determinant of mAb cost. 

 

mAb purification, largely reliant on chromatography (e.g. Protein A chromatography), is the principal determinant of drug cost. The present invention generally relates to non-chromatographic purification of mAbs, and particularly, to a method of purification of monoclonal antibodies via selective coacervation with hyaluronic acid. The method separates mAb from unwanted proteins and nucleic acids secreted from mAb-producing hybridoma cells, forming a fluid coacervate from which the mAb is purified. This promising technique may be an effective alternative for Protein A chromatography.

Published: 8/10/2017   |   Inventor(s): Paul Dubin, Rachel Wollacott, Daniel Seeman, Alex Malanowski
Category(s): Biotechnology, Healthcare, Life Sciences, Therapeutics & prevention
Inhibiting Proteases to Accelerate Wound Repair
This invention relates to the use of a protein therapeutic to block the activated and over-expressed proteases that lead to chronic wounds and thinning of the dermis in aging.
Published: 9/8/2016   |   Inventor(s): Annette Wysocki, Margaret Riley
Category(s): Healthcare, Life Sciences, Therapeutics & prevention
Nanoparticle-Stabilized Microcapsules for Effective Treatment of Bacterial Biofilms
Bacterial biofilms are widely associated with persistent infections. The amphiphilic construct of biofilms provides protection for bacterial cells by reducing absorption of conventional antimicrobials. This invention provides new nanoparticle-stabilized antimicrobial microcapsules that can effectively inhibit and eradicate pathogenic biofilms. The microcapsules contain antimicrobial essential oil materials and can efficiently deliver such materials to the cells of pathogenic bacteria in the biofilm, resulting in effective killing of the bacteria.
Published: 10/5/2015   |   Inventor(s): Vincent Rotello, Bradley Duncan, Xiaoning a/k/a Sharlene Li
Category(s): Healthcare, Life Sciences, Nanotechnology, Therapeutics & prevention
Polymeric Nanocapsules for the Treatment of Biofilms
Bacterial biofilms are widely associated with persistent infections. The amphiphilic construct of biofilms provides protection for bacterial cells by reducing absorption of conventional antimicrobials. This invention provides new antimicrobial nanocapsules that can effectively inhibit and eradiate pathogenic biofilms. The nanocapsules contain an antimicrobial essential oil and can efficiently deliver the essential oil to the cells of pathogenic bacteria in the biofilm, resulting in effective killing of the bacteria.
Published: 9/14/2015   |   Inventor(s): Vincent Rotello, Ryan Landis, Akash Gupta, Yiwei Lee
Category(s): Biotechnology, Healthcare, Life Sciences, Nanotechnology, Therapeutics & prevention
Polymeric Nanocapsules for Efficient Intracellular Delivery of Theraputic Proteins
Designing protein therapeutics is becoming one of the fastest growing strategies to treat a variety of diseases including cancer and diabetes. Clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9, a bacterially constructed protein-nucleic acid complex has shown great potential to reverse various genetic disorders. The delivery of this complex into the cell and finally the nucleus is challenging, however, due to the large size of the Cas9 protein. This invention provides a general and efficient protein delivery platform that enables the intracellular delivery of therapeutic proteins. The delivery system involves the use of novel synthetic polymers capable of forming nanocapsules spontaneously under controlled conditions.
Published: 6/22/2015   |   Inventor(s): Vincent Rotello, Ryan Landis, Moumita Ray
Category(s): Biotechnology, Life Sciences, Research tools, Healthcare, Therapeutics & prevention
Efficient Cytosolic Delivery of siRNA Using Nanoparticle-Stabilized Nanocapsules
This invention provides a general and efficient protein delivery platform that enables intracellular delivery of proteins having different physiochemical properties. The delivery system involves the use of surface functionalized nanoparticles to form self-assembled superstructures with the protein to be delivered. The nanoparticle-protein assemblies effectively escape endosomal entrapment and rapidly deliver the protein into the cell cytosol or the targeting organelle. This protein delivery system has been successfully demonstrated for the efficient delivery of the CRISPR/Cas9 gene editing system as well as a number of other proteins with different physiochemical properties.
Published: 6/19/2015   |   Inventor(s): Vincent Rotello, Ying Jiang, Rui Tang
Category(s): Biotechnology, Life Sciences, Healthcare, Research tools, Therapeutics & prevention
Semprevivo/ Lipoglycan Carbohydrate Fragment-Protein Conjugate Vaccine for Schistosomiasis
Two new vaccines have been developed based on an

 

understanding of host immune responses and how the parasite survives in the immunologically

 

complete host. The new vaccinations utilize the parasite’s own protection mechanism by inducing an IgG immunological response to remove or prevent Schistosoma and Fasciola infections. Laboratory tests in mice have shown 100% effectiveness against Schistosoma and Fasciola virulent challenge, and a preliminary double blind cattle trial conducted in South Africa generated a protection of 75%.

 

Published: 5/27/2015   |   Inventor(s): Lloyd Semprevivo
Category(s): Therapeutics & prevention, Veterinary science
Bacterial Polysaccharides for Inflammatory Disease
Inflammation is a healthy biological response which serves to remove harmful irritants and promote healing. However, abnormal inflammation may lead to pain, suffering, and disease, including digestive disease and autoimmune disease (e.g. multiple sclerosis). Exopolysaccharides (EPS) are polymers made up of sugars residues and are secreted by bacteria. The present invention involves administration of bacterial EPS to prevent and protect against inflammatory disease. Using animal models, the inventors have found that administration of Bacillus subtilis EPS formulations prevent intestinal inflammation. In addition, these formulations prevent development of a multiple sclerosis-like disease in mice. Thus, Bacillus subtilis EPS could be used as a therapeutic or serve as a prophylactic for inflammatory disorders, and thereby improve the quality of life for individuals with these diseases.
Published: 4/30/2015   |   Inventor(s): Barbara Osborne, Furkan Ayaz, Katherine Knight, Sara Jones
Category(s): Biotechnology, Healthcare, Life Sciences, Therapeutics & prevention
An Efficient Intracellular Delivery Vehicle for the CRISPR/Cas9 Gene Editing System and Other Proteins
This invention provides a general and efficient protein delivery platform that enables intracellular delivery of proteins having different physiochemical properties. The delivery system involves the use of surface functionalized nanoparticles to form self-assembled superstructures with the protein to be delivered. The nanoparticle-protein assemblies effectively escape endosomal entrapment and rapidly deliver the protein into the cell cytosol or the targeting organelle. This protein delivery system has been successfully demonstrated for the efficient delivery of the CRISPR/Cas9 gene editing system as well as a number of other proteins with different physiochemical properties.
Published: 4/27/2015   |   Inventor(s): Vincent Rotello, Rubul Mout
Category(s): Biotechnology, Healthcare, Life Sciences, Research tools, Nanotechnology, Therapeutics & prevention
Amphiphilic Polymer Capsules and Related Methods of Interfacial Assembly
A chemical crosslinking process is used to prepare capsules from PEGylated polyolefins using either oil-in-water (i.e., oil inside the capsule in a water-based system), or water-in-oil system. The covalent network structure of these capsules make them more robust than many other systems under investigation in controlled-release.The unique nature of the crosslinking chemistry is such that the crosslinks can be made either hydrolytically stable or unstable. Those with hydrolytically stable crosslinks have longer carrier lifetimes, while those with hydrolytically unstable crosslinks will degrade over a time period that can be controlled by crosslink density and the type of crosslinker used.
Published: 12/1/2014   |   Inventor(s): Kurt Breitenkamp, Todd Emrick
Category(s): Material science, Therapeutics & prevention, Biotechnology
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